Four of the controlled

studies combined VAE and conventional cancer treatment. These studies partly reported a benefit regarding disease recurrence and time to disease relapse and partly no difference; none found a disadvantage. Two single-arm studies reported tumour remission in 44–62% Enzalutamide research buy of patients after local application of high dosage VAE. Another study found no remission after the application of rML. QoL and the reduction of side effects of chemotherapy, radiation and surgery (Tables 5 and 6) were assessed by 11 RCTs, 6 non-RCTs and 4 single-arm studies: 19 of these 21 studies reported a benefit, mostly statistically significant, one study reported no QoL-benefit but a reduction of side effects, and the smallest of these studies found no difference. Three major pharmaco-epidemiological studies investigated patient charts and found reduced disease- and therapy-associated symptoms in VAE-treated groups. In preclinical studies (Tables 7, 8, and 9) VAE and VAE compounds showed cytotoxic effects in cancer cells. VAE also counteracted

growth factor-induced proliferation and migration in breast cancer cells [95]. In mice, VAE inhibited tumour NVP-HSP990 ic50 growth in most cases, especially when applied locally and in high dosage. Survival was prolonged in most cases, and numbers of metastases and local NU7026 recurrences were reduced after application of VAE or of VAE-activated macrophages; Tenoxicam one study found no benefit. All experiments using local VAE application found a benefit in relation to survival and tumour-growth inhibition. In rats, no clear benefit of VAE could be seen. Results from applying isolated or recombinant VAE compounds were inconsistent: some moderate effects of proteins (e.g. lectins) or polysaccharides were observed in relation to survival and tumour growth, while others

observed none or possibly also adverse outcomes. Cervical cancer   Clinical studies: Survival (Table 3) was investigated by one RCT and three non-RCTs: all four reported a beneficial outcome which, however, was statistically significant only in the non-RCTs. Tumour behaviour (Table 4) was investigated by one non-RCT, which could not find an effect on disease recurrence or metastases mainly because these events scarcely occurred. One single-arm study reported 41% complete and 27% partial remissions in CIN after VAE application. QoL (Table 5) was assessed in one RCT and one non-RCT; both reported a statistically significant benefit. Regarding preclinical studies (Table 7), only HeLa cells were investigated; here VAE and protein fractions showed cytotoxic effects. Uterus cancer   Clinical studies: Survival (Table 3) was investigated by two RCTs and two non-RCTs; three reported a statistically significant benefit while one found no difference. QoL (Table 5) was assessed by one RCT and one non-RCT; both found a statistically highly significant benefit.