3B-h). To further dissect how AR regulates MMP-9 at the transcriptional level, we constructed an MMP-9 promoter (ranged from
+2 to −2629) hooked with a luciferase vector to test whether AR could negatively regulate MMP-9 promoter transactivation activity, and found that AR could suppress MMP-9 expression in promoter regulation (Supporting Fig. 7A-C). We also applied the zymography assay to detect MMP-9 activity, and found higher Ceritinib in vitro MMP-9 proteolytic activity in ARKO BM-MSCs, compared with WT BM-MSCs (Fig. 3B-i). This was also confirmed in studies using hMSCs manipulated with AR-siRNA (Supporting Fig. 6C,F). To test whether ARKO-mediated enhanced migration is MMP-9 dependent, we pretreated ARKO BM-MSCs with an MMP-9 inhibitor and performed the migration assay, and results showed that the addition of the MMP-9 inhibitor indeed masked ARKO-mediated enhanced migration ability (Fig. 3B-j), suggesting that AR needs to go through MMP-9 to exert its influence on BM-MSC migration. Together, results from three different types of assays all proved that MMP-9 is a critical molecule to mediate the enhanced www.selleckchem.com/products/ganetespib-sta-9090.html migration ability of ARKO BM-MSCs. Finally, we confirmed the above-described findings showing KO of AR in BM-MSCs increased self-renewal potential and migration capacity in CCl4-induced liver cirrhotic mice. Consistently, ARKO BM-MSCs-transplanted liver showed higher Ki67/GFP double-positive
stained cells (representing proliferating transplanted BM-MSCs) than WT BM-MSCs (Fig. 3C-k-m). To correlate the increased self-renewal and migration potentials of ARKO BM-MSCs improvement in anti-fibrosis and anti-inflammatory 上海皓元 actions, we used conditioned medium (CM) of BM-MSCs to test their effects on macrophage migration and HSCs proliferation. Results showed that BM-MSCs-inhibited macrophage migration (anti-inflammatory effects) and HSCs proliferation (anti-fibrotic actions) were BM-MSCs-number dependent (Fig. 3D,E), suggesting
that KO of AR-increased self-renewal and migration of BM-MSCs resulted in more BM-MSCs to exert better anti-inflammation and anti-fibrotic actions. Together, results (from Fig. 3A-E) concluded that KO of AR in BM-MSCs led to increased self-renewal and migration potentials of BM-MSCs and these resulted in better transplantation therapeutic efficacy to treat liver cirrhosis by exerting better anti-fibrotic and anti-inflammatory effects. These phenotypes were involved in the modulation of EGF-Erk1/2/Akt signals, as well as MMP-9 signals. All above-described results demonstrated that higher numbers of BM-MSCs migrating into the cirrhotic liver led to better transplantation therapeutic efficacy with higher anti-inflammatory and anti-fibrotic effects (Fig. 3D,E). We were interested to know whether there are any secreted paracrine factors influenced by knockout of AR in BM-MSCs to contribute to anti-inflammatory and -fibrotic actions.