alleles Simpson’s Unique SNP SNP/allele (average) Mutations per allele (average) dN/dS
ID CI (95%) Silent Conserv. Missense Nonsense Average St. dev. blaZ 54 11 79.2 69.6-88.8 41 11.4 5.3 1.7 3.7 0.1 0.21 0.11 MRSA blaI 27 7 82.1 74.6-89.5 10 3.9 2.9 0 1.0 0 0.11 0.05 blaR1 31 10 88.8 83.2-94.4 60 24.4 9.7 5.3 8.0 0 0.24 0.11 blaZ 24 9 76.1 61.3-90.9 35 14.7 7.1 1.9 4.6 0 0.17 0.04 MSSA blaI 20 6 74.2 60.5-87.9 9 2.5 1.5 0.2 0.8 0 0.08 0.03 blaR1 17 8 88.2 81.2-95.3 61 24.6 10.4 5.5 7.8 0 0.24 0.10 blaZ 78 13 81.1 75.0-87.3 43 12.4 5.8 1.8 4.0 0.1 0.20 0.10 All blaI 47 9 78.4 71.0-85.9 13 3.4 2.3 0.1 1.0 0 0.10 0.04 blaR1 OTX015 mouse 48 12 88.5 84.0-93.0 65 24.8 10.2 5.3 8.1 0 0.25 0.10 ID, index of diversity; CI, confidence interval; SNP, single-nucleotide polymorphism; Conserv., Apoptosis Compound Library mw conservative; St. dev., standard deviation Figure 1 blaZ allotype frequency per MRSA lineage as defined
by MLST clonal cluster. Figure 2 Cluster tree of blaZ gene allotypes found in the MRSA and MSSA collections. The tree was constructed with the neighbor-joining (NJ) method. In each branch is shown the corresponding bootstrap NJ values, taken over 1000 replicates, which assigns confidence values for the groupings in the tree. For each allele, it is indicated the collection(s) (MRSA or MSSA) and genetic lineage (clonal cluster) where it was found. The BlaZ variability in the MRSA and MSSA strains at the protein level was evaluated by comparison of the deduced amino acid sequence of all alleles against the deduced amino acid sequence for the BlaZ of Tn552. Overall, the deduced amino acid sequences of blaZ alleles from the MRSA and MSSA strains revealed on average 5.8 silent mutations, 1.8 conservative missense mutations and 4 non-conservative missense mutations per allotype (see Tables 3 and 4). For MRSA strain HAR40, a nonsense mutation at Gln76 was detected which presumably originates a non-functional truncated BlaZ protein. As this strain was positive for the nitrocefin test, the DNA extraction and the blaZ sequencing were repeated and the nonsense mutation was confirmed.
No frameshift mutations were found in blaZ allotypes. Allelic variability of blaZ regulatory genes Based on the blaZ variability analysis, we selected 51 representative strains to further characterize the variability in the blaZ selleck inhibitor regulatory genes, blaI and blaR1. Some of these strains failed in the amplification of one of the blaZ regulatory genes (see Tables 1 and 2). Within the length of blaI region analyzed (351 nucleotides), we detected 13 unique SNP, which account for the nine blaI allotypes detected (see Tables 3 and 4).