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26. Navarre WW, Porwollik S, Wang Y, McClelland M, Rosen H, Libby SJ, Fang FC: Selective silencing of foreign DNA with low GC content by the H-NS protein in Salmonella. Science 2006,313(5784):236–238.CrossRefPubMed EPZ5676 nmr Authors’ contributions DZ and RY conceived the study and designed the experiments. LJZ and LY performed all the experiments. LZ, YL and HG contributed to RT-PCR, primer extension assay and DNA binding assays. ZG participated in protein expression and purification. DZ, LFZ, CQ and DZ assisted in computational analysis and figure construction. The manuscript was written by LJZ and DZ, and revised by RY. All the authors read and approved the final manuscript.”
“Background Salmonellae are gram-negative bacteria selleck chemicals causing a variety of disease syndromes in humans and animals. For example, Salmonella enterica serovar Typhi causes a systemic disease in human known as typhoid fever, whereas S. enterica serovar Glutathione peroxidase Typhimurium is responsible for gastroenteritis in humans and a systemic disease in mice similar to human typhoid fever. The ability of Salmonellae to survive within macrophages is required for systemic

disease [1]. Important virulence factors are introduced into the host environment including the host cell cytosol using two different type III secretion systems (TTSSs) encoded on the Salmonella pathogeniCity islands, SPI-1 and SPI-2 [2]. SPI-1 TTSS mediates bacterial entry into non-phagocytic cells [3] and SPI-2 TTSS is required for survival and replication in the intracellular environment of host cells and contributes to systemic infection in animals [4–6]. The spiC gene is adjacent to spiR (ssrA)/ssrB, a two-component regulatory gene, and is the initial gene for the operons encoding the structural and secretory components of SPI-2 [4]. Previous studies show that a strain carrying a mutation in the spiC gene is unable to survive within macrophages and has greatly reduced virulence in mice. The SpiC protein is necessary to inhibit the fusion of Salmonella-containing phagosomes with endosomal and lysosomal compartments [7]. SpiC is translocated by SPI-2 TTSS to the cytosol of the macrophages where it interacts with host proteins, i.e.