The online version contains additional product offered at 10.1007/s13205-021-02842-4.Algae have gained considerable value since the many encouraging possible green gas resource around the world and it is on developing need due to their anti-oxidant, anticancer, antiviral, antihypertensive, cholesterol relieving and thickening properties. Therefore, it has huge selection of application in drugs, pharmaceutical, cosmetics, paper and nutraceutical sectors. In this work, the remarkable ability of algae to convert CO2 as well as other toxic compounds in atmosphere to prospective biofuels, foods, feeds and high-value bioactive compounds is reviewed. Algae create around 50% of this planet’s air having its photosynthetic activity, therefore acting as a potent device to mitigate the consequences of polluting of the environment. Further, the applicability of algae as a desirable energy source has also been discussed, as they possess possible to act as a powerful option to intermittent renewable energy; also, to combustion-based fossil fuel power, making them efficient for advanced biofuel conversion rates. This work additionally evaluates the current programs of algae in addition to implications of it as a possible substrate for bioplastic, all-natural alternative to selleck products inks and for making report besides high-value services and products. In inclusion, the scope for built-in biorefinery strategy can also be fleetingly investigated when it comes to financial aspects during the industrial scale, as such energy transformation components tend to be right linked with sustainability, hence offering an optimistic total energy outlook.D-pantothenic acid (D-PA), as an important supplement, is trusted in food, animal feed, cosmetics, and pharmaceutical industries. In our earlier work, recombinant Escherichia coli W3110 for production of D-PA had been constructed through metabolic path adjustment Immunoprecipitation Kits . In this study, to enhance D-PA production, statistical optimization practices including Plackett-Burman (PB) design and Box-Behnken design (BBD) initially were used to enhance the tradition problem. The outcome showed that the glucose, β-alanine and (NH4)2SO4 possess most critical impacts on D-PA biosynthesis. The response surface model according to BBD predicted that the optimal concentration is glucose 56.0 g/L, β-alanine 2.25 g/L and (NH4)2SO4 11.8 g/L, the D-PA titer increases from 3.2 g/L to 6.73 g/L shake flask fermentation. For the fed-batch fermentation in 5 L fermenter, the isoleucine feeding strategy greatly increased the titer and efficiency of D-PA. As a result, titer (31.6 g/L) and output (13.2 g/L·d) of D-PA had been achieved, they enhanced by 4.66 times and 2.65 times, correspondingly, compared with batch culture. As well, the accumulation of acetate reduced from 29.79 g/L to 8.55 g/L when you look at the fed-batch fermentation. These results demonstrated that the optimization of method structure therefore the cellular growth price are very important to increase the focus of D-PA for microbial fermentation. This work laid the building blocks for further analysis regarding the application of D-PA microbial synthesis.The internet variation contains supplementary immunoregulatory factor product offered at 10.1007/s13205-021-02773-0.Cyst nematodes associated with the species Globodera rostochiensis and G. pallida are damaging parasites associated with potato crop. Early detection of cyst nematodes on the go is important for adopting a proper management strategy. A particular and painful and sensitive loop-mediated isothermal amplification (LAMP) assay utilizing four oligonucleotide primers has been created to amplify the inner transcribed spacer region (ITS) of ribosomal DNA of potato cyst nematode G. rostochiensis. The PCN-LAMP reaction could possibly be completed within 75 min at 68 °C accompanied by cancellation at 85 °C for 7 min. The primers exhibited specificity for G. rostochiensis and did not identify any kind of tested genera of plant parasitic or entomopathogenic nematodes. LAMP reaction was extremely painful and sensitive, suitable for crude genomic DNA and may effectively detect G. rostochiensis DNA up to femtogram volume. This assay is fast, affordable and requires minimal instrumentation. It will facilitate the detection of G. rostochiensis at field and point-of-care labs and help in the interception of infested plant material/soil samples at quarantine programs separate of a specialist nematologist.The internet version contains additional material available at 10.1007/s13205-021-02830-8.Conventional agricultural practices based on the application of synthetic fertilizers tend to be more and more considered as unsustainable. Under a forecasted situation of drought for the following decades, there is an international demand for innovative and sustainable methods to ameliorate plant performance. Here, encapsulating advantageous microbes (BMs) to promote plant growth is gaining interest. This research evaluates bacterial encapsulation utilizing polymeric beads of alginate, testing the success of Pseudomonas libanensis TR1 stored up to 90 days. Produced beads were subjected to different treatments (fresh, air-dried and pulverized), which lead to a variable size range (1200-860 µm). After storage, microbial viability was preserved, and air-dried beads exhibited an increased amount of colony-forming units (2 × 107). Then, a glasshouse experiment investigated the drought opposition (plant growth, biomass, and photosynthetic responses) of Vigna unguiculata plants inoculated with one of these alginate beads. After 10 times of complete liquid limitation, turgidity and relative liquid content of V. unguiculata remained high under drought stress (> 80%). Leaf and root development and biomass didn’t research considerable modifications after water limitation even after P. libanensis inoculation. Plant photosynthetic variables (stomatal conductance, net photosynthetic rate, leaf CO2 concentration, or F v’/F m’) were slightly impacted as a result of inoculation nevertheless the level of stress-induced minimal plant responses.