Figure 5 shows the location in the dcw and SpoIIG clusters of these putative terminators. The DNA sequences that form the structures are shown

below the drawing. They are 100% identical in DX and in B. weihenstephanensis. Six out of seven are assigned a 100% confidence score by the algorithm of the program, and the seventh, between sigmaE and sigmaG, has an 89% score. The SIN termination structures are not identical, but maintain the characteristic of SB203580 in vivo terminators with one or a few different nucleotides, the same level of diversity existing for instance between the terminators of B. weihenstephanensis and those of B. anthracis Ames. Figure 5 Transcriptional terminators within the B. mycoides dcw and spoIIG gene clusters. Red labels mark the position of the putative terminators. The DX termination sequences displayed are 100% identical to those MS-275 in vitro predicted at the

TransTerm-HP site for B. weihenstephanensis selleck products KBAB4 (Accession NC_010184, from coordinates 3780796 to 3790953). The green label between ftsA and ftsZ indicates a hairpin structure not recognized there as a potential terminator. The three large green bars over the genes represent the main ftsZ-specific RNAs and the green thin bars the minor ones. The primers used to detect RNA 5’ ends by primer extension are indicated below the genes. The curved arrows in the enlarged region show the main ftsA and ftsZ RNA start sites. The short 39 bp DNA region between ftsA and ftsZ can also be folded into a hairpin

structure with a calculated stability of −7.8 ΔG, though it is not recognized as a potential terminator by the TransTerm-HP site and is tagged with a different color in the figure. Downstream of the dcw cluster, in the group composed of three genes, SpoIIA-sigmaE processing peptidase, prosigmaE and Hydroxychloroquine sigmaG, putative termination sequences are located between prosigmaE and sigmaG and after sigma G, at the end of the group. The putative terminators are located at the boundary between genes of different specificity, which code either for enzymes of peptidoglycan biosynthesis or for structural proteins of the division septum, meaning that terminators are found between the mur/fts genes and not between the mur/mur or fts/fts genes. Two consecutive terminator hairpins close the dcw cluster immediately after the ftsZ gene. In B. anthracis, another member of the B. cereus group, the genome-wide coverage of DNA by RNA transcripts has been analyzed at the single nucleotide level [7]. The high-throughput sequencing of total RNA (RNA-Seq), in various growth conditions, provided a map of transcript start sites and operon structure throughout the genome. Discontinuity of RNA transcripts in B.