The ability of Ts-DF venom to induce more potent effects on secretory discharge as well as on vesicular transport mechanisms in the exocrine pancreas than Ts-MG venom needs to be verified. In conclusion, the observation of a smaller diversity of supposedly NaScTx in the Ts-DF venom may explain the lower toxicity of this venom when compared to Ts-MG. Also, the inability to induce acute pulmonary edema in rats could explain the absence of severe clinical symptoms and death in patients stung by scorpions

in the DF. Given the above Trametinib and considering the LD50 determined here, it can be inferred that the maximum amount of venom that could be inoculated by T. serrulatus from the DF during a sting would not be sufficient to induce the onset of symptoms of severe poisoning in humans, while the T. serrulatus scorpion releases about 450 μg of venom after electrical stimulation (data not shown). However it is noteworthy that scorpionism in the DF cannot be neglected because of the increased presence of T. serrulatus in this region. This can over time trigger the emergence of a public health problem. Financial support: FAPDF, CNPq

(306524/2012-0 and 564223/2010-7 to EFS and 308929/2011-0 to AMCP), PPG BioMol-UnB, CAPES, FAPEMIG and MCT-FINEP. Fagner Neves Oliveira (579427/2008-0) and Jimmy A. Guerrero Vargas (553137/2007-7) also received fellowship from CNPq. The authors greatly acknowledge Natiela B. de Oliveira, Caroline B. F. Mourão, Braulio S. S. Filho and Lucélia G. for Vieira for technical assistance. Jimmy A. Guerrero-Vargas is member of Grupo de Investigaciones Herpetologícas y Toxinológicas from Universidad del Cauca, Colombia. “
“Spiders Ruxolitinib clinical trial of the genus Loxosceles, commonly known as brown spiders, have a worldwide distribution with more than 100 species present in Europe, Africa, Oceania, Asia, North America, Central America, and South America ( Vetter, 2008). In Brazil, especially in the southern and southeastern regions, the predominant species are Loxosceles intermedia, Loxosceles gaucho, and Loxosceles laeta ( Pauli et al., 2006). Over

the last decade, research studies, motivated by the growing number of envenomation cases have reported that the spider distribution has become heterogeneous and includes urban areas ( da Silva et al., 2004 and Hogan et al., 2004; Chatzaki et al., 2012; Tambourgi et al., 2010). Envenomation cases in humans are characterized by two clinical manifestations: cutaneous and systemic loxoscelism. The former is characterized by the formation of a dermonecrotic lesion. The second, which is also known as cutaneous-visceral loxoscelism, presents clinical manifestations that may cause, in some situations, disseminated intravascular coagulation, acute renal failure and in rare cases, generalized rash and death (Futrell, 1992 and Swanson and Vetter, 2005). Several protocols for the treatment of Loxosceles envenomation have been proposed and tested.