The determination of the viability of each group of microorganisms and of the metabolic activity was performed for each simulated compartment. In order to confirm the biochemical tests, at least one colony from each sample was analyzed from a genetic point of view, using the repetitive-element
PCR fingerprinting (rep-PCR) technique. Molecular analysis demonstrated the persistence of the two strains representing the basis of the PROBAC product, and the genetic stability of the lactic acid bacteria strains included in the final product after transiting Selinexor the various parts of the simulated colon. After analyzing the viability of lactic acid, bacteria strains that were introduced in the final product in the presence and absence of the prebiotic while transiting the sections of
the colon, produced an increase in lactic and bifidobacteria number, especially in the transverse and descending sections. In vitro approaches to study the human colon microbiota, and intestinal microbial processes offer an excellent experimental set-up to study the mechanism of action.”
“Introduction: Many experimental models using lung lavage have been developed for the study of acute respiratory distress syndrome (ARDS). The original technique has been modified by many authors, resulting in difficulties with reproducibility. There is insufficient detail on the lung injury models used, including hemodynamic stability during animal preparation and drawbacks encountered such as mortality. The authors studied the effects of the pulmonary recruitment and the use of fixed BKM120 mw DAPT solubility dmso tidal volume (Vt) or fixed inspiratory pressure in the experimental ARDS model installation.
Methods: Adult rabbits were submitted to repeated lung lavages with 30 ml/kg warm saline until the ARDS definition (PaO2/FiO(2) <= 100) was reached. The animals were divided into three groups, according to the technique used for mechanical ventilation: 1) fixed Vt of 10 ml/kg; 2) fixed inspiratory pressure (IP) with a tidal volume of 10 ml/kg prior to the first lung lavage; and 3) fixed
Vt of 10 ml/kg with pulmonary recruitment before the first lavage.
Results: The use of alveolar recruitment maneuvers, and the use of a fixed Vt or IP between the lung lavages did not change the number of lung lavages necessary to obtain the experimental model of ARDS or the hemodynamic stability of the animals during the procedure. A trend was observed toward an increased mortality rate with the recruitment maneuver and with the use of a fixed IP.
Discussion: There were no differences between the three study groups, with no disadvantage in method of lung recruitment, either fixed tidal volume or fixed inspiratory pressure, regarding the number of lung lavages necessary to obtain the ARDS animal model.