Vegetative hyphae were added directly to slides coated with 1% (w/v) agarose in phosphate-buffered

saline. Spore chains were collected by pressing coverslips on the surface of colonies and then placing them on agarose-coated slides. Images of fluorescence signals were captured and analysed quantitatively using a previously described microcopy system [30]. Aerial mycelium and spores of all mutants were also investigated by phase-contrast microscopy. Heat resistance of spores The ability of spores to survive incubation at 60°C was assayed as described previously [30]. www.selleckchem.com/products/AC-220.html Availability of supporting data The microarray data has been deposited with ArrayExpress (Accession number: E-MTAB-1942). Acknowledgements This work was supported by postdoctoral stipends from Carl Tryggers Foundation to PS and NA, and by grants from the Swedish Research Council (No. 621-2007-4767) to KF and the European Commission FP6 Programme,(No, IP005224, ActinoGEN) to CPS. Electronic supplementary

material Additional file 1: Table S1: Genes that are differentially expressed when comparing whiA or whiH mutant to the wild-type parent, or comparing the developing wild-type strain at 36 h or 48 h to the expression pattern at 18 h. All ORFs having an adjusted p-value <0.05 in at least one of the eight comparisons (A18, A36, A48, H18, H36, H48, wt36, wt 48) are listed. There Epigenetic Reader Domain inhibitor are 285 ORFs in total. (XLSX 47 KB) Additional file 2: Contains Additional Ureohydrolase files: Figure S1-S5 and their legends. (PDF 3 MB) Additional file 3: Table S2: Oligonucleotide primers used in this study. (PDF 2 MB) References 1. Chater KF: Differentiation in Streptomyces : the properties and programming of diverse cell-types. In Streptomyces: Molecular Biology and Biotechnology. Edited by: Dyson P. Norfolk, UK: Caister Academic Press; 2011:43–86. 2. Flärdh K, Buttner MJ: Streptomyces morphogenetics: Dissecting differentiation in a filamentous bacterium. Nat Rev Microbiol 2009,

7:36–49.PubMedCrossRef 3. Chater KF, Biro S, Lee KJ, Palmer T, Schrempf H: The complex extracellular biology of Streptomyces . FEMS Microbiol Rev 2010,34(2):171–198.PubMedCrossRef 4. McCormick JR, Flärdh K: Signals and regulators that govern Streptomyces development. FEMS Microbiol Rev 2012,36(1):206–231.PubMedCentralPubMedCrossRef 5. Van Wezel GP, McDowall KJ: The regulation of the secondary metabolism of Streptomyces : new links and experimental advances. Nat Prod Rep 2011,28(7):1311–1333.PubMedCrossRef 6. Bibb MJ, Domonkos A, Chandra G, Buttner MJ: Expression of the chaplin and rodlin hydrophobic sheath proteins in Streptomyces venezuelae is controlled by sigma(BldN) and a cognate anti-sigma factor, RsbN. Mol Microbiol 2012,84(6):1033–1049.PubMedCrossRef 7. Den Hengst CD, Tran NT, Bibb MJ, Chandra G, Leskiw BK, Buttner MJ: Genes essential for morphological development and antibiotic production in Streptomyces coelicolor are targets of BldD during vegetative growth. Mol Microbiol 2010,78(2):361–379.