We reasoned that if survivin plays a role in bortezomib resistance, p53 status might affect bortezomib sensitivity to inhibit
cancer cell growth. Consistent with our rationale, p53 null www.selleckchem.com/products/3-deazaneplanocin-a-dznep.html HCT116 cells (HCT116p53-/-) were resistant to bortezomib-induced cell growth inhibition in comparison with HCT116 with wild type p53 (Fig 1). This suggests a role for the p53 status in bortezomib-induced cancer cell growth inhibition, however it is not known whether the difference of p53 status can Bafilomycin A1 in vivo also affect bortezomib-induced cell death. Figure 1 Colon cancer cell growth inhibition by bortezomib. HCT116 colon cancer cells with p53 wild type (p53+/+) and p53 null (HCT116p53-/-) were treated with bortezomib at different concentrations for 48 hours. Cell growth was determined by MTT assay (A) or by direct cell counting (B). The resultant data were plotted in histogram by setting no bortezomib treatment controls as OD values at 1 (A) or as cell numbers at 100. Each bar represents the mean ± SD derived from three independent determinations. HCT116p53-/- colon cancer cells are much more resistant to bortezomib-mediated cell death in comparison with wild type HCT116 cells We then tested the effect of bortezomib
on the induction of HCT116 colon cancer cell death with different p53 status. Flow cytometry was used to determine DNA content profiles as a parameter to evaluate cell death after bortezomib treatment. This experiment revealed that bortezomib treatment for 24 hours at 10 and 50 nM significantly induced sub-G1 DNA (representing dead cells) content increase find more in HCT116p53+/+ cells, while this treatment showed minimal effect on HCT116p53-/- cells (Fig. 2). These observations (Figs 1 and 2) prompted us to investigate the potential role for survivin in bortezomib resistance. Figure 2 Colon cancer cell death induced by bortezomib. Sub-confluent HCT116 and HCT116p53-/- cells were treated with and without bortezomib at different concentrations as shown
for 24 hours. Cells were then collected and stained with PI, followed by flow cytometry analysis of DNA content profiles. A. The flow cytometry resultant data in histogram showed the striking difference in DNA content profiles 4-Aminobutyrate aminotransferase between HCT116 cells and HCT116p53-/- cells. B. Histogram to compare the different percentage of cells in sub-G1 (dead cells) between HCT116 cells and HCT116p53-/- cells. The histogram in B is the mean ± SD derived from two independent determinations. Survivin expression is much higher in HCT116p53-/- cells than in HCT116p53+/+ cells We reasoned that if survivin plays a role in bortezomib resistance, survivin expression would be lower in HCT116p53+/+ cells than in HCT116p53-/- cells. Alternatively, bortezomib may decrease survivin expression in HCT116p53+/+ cells or increase survivin expression in HCT116p53-/- cells.