One-way ANOVA was used as appropriate to analyze rER variances of areas (I, O, and C) within each survival group. Differences between individual bone forming areas within samples were analyzed with paired t-tests. Differences between isotransplants and allotransplants and between survival periods were compared

with unpaired t-tests. Data are presented as mean ratio with standard deviation. Significance is Y-27632 concentration set at p < 0.05. In all animals, the pedicle was patent at inspection of polymer filling of the vasculature. The rER in allotransplants at 4 weeks (A) was 0.456 ± 0.266 in the overall cortical area, while it was slightly higher at the outer cortex; 0.549 ± 0.184 and lower at the inner cortex; 0.362 ± 0.081. The rER at 18 weeks (group B) had increased in all areas, with an overall cortical rER of 0.749 ± 0.387; however, this difference did not reach significance (p > 0.05). The rER GSK2126458 mw at the inner cortex at 18 weeks was 0.532 ± 0.188, at the outer cortex 0.586 ± 0.175 (Table

1). In the isotransplant group at 4 weeks (group C), the overall cortical rER was 0.412 ± 0.239. The inner cortex had a rER of 0.398 ± 0.241, while at the outer cortex the rER was 0.247 ± 0.181. At 18 weeks in isotransplants (group D), the overall rER was slightly higher 0.467 ± 0.252 than group C (p > 0.05), with an inner cortex rER of 0.356 ± 0.113 and an outer stiripentol cortex rER of 0.392 ± 0.229. The short-term survival groups (A and C) had a comparatively equal overall cortical rER. At 18 weeks, the rER was higher in allotransplants (group B) as compared to the isotransplants (group D); however, no statistical significant difference was found (p > 0.05). At the outer cortical areas, the rER was significantly lower at 4 weeks in isotransplants

as compared to allotransplants (p < 0.05). This difference at the outer cortex was not found at 18 weeks. In the allotransplant group, a slight increase over time was found at the inner cortex, while in isotransplants, the rER remained lower than 0.5 over time with a majority of cells of donor origin. For successful incorporation and optimal biological properties of bone grafts, remodeling is a prerequisite. To understand the biology behind this process, knowledge of cellular heritage and the movement of cells in the transplant over time is essential. We applied a sex-mismatch rat model that has been used successfully in our previous bone transplantation research.[15-17] This transplantation model allows the study of cell lineage with quantitative RT-PCR on the Sry and cyclophilin housekeeper genes to detect the relative amount of recipient cells to donor cells within the transplant. Laser capture microdissection facilitates highly selective harvest of tissue, without contamination of adjacent soft tissue including capillary tissue.